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rosettesep method  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc rosettesep method
    Rosettesep Method, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rosettesep+method/rosettesep+method/pmc11370957-102-5-7
    Average 90 stars, based on 1 article reviews
    rosettesep method - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Liquid biopsy: a step closer to transform diagnosis, prognosis and future of cancer treatments
    Article Snippet: Other examples, like the RosetteSep (StemCell Technologies) method, use an additional density gradient centrifugation step for further CTC enrichment [ ].

    Article Title: Strategies for enrichment of circulating tumor cells.
    Article Snippet: For example, the RosetteSep method, which was developed by StemCell Technologies (Vancouver, Canada) , combines densi ty gradient centrifugation with negative enrichment (76-78).

    Article Title: Strategies for enrichment of circulating tumor cells
    Article Snippet: For example, the RosetteSep method, which was developed by StemCell Technologies (Vancouver, Canada), combines density gradient centrifugation with negative enrichment ( - ).

    Article Title: Technical challenges in the isolation and analysis of circulating tumor cells
    Article Snippet: One notable example is the RosetteSep method from StemCell Technologies (Vancouver, Canada) [ ].

    Gradient Centrifugation:

    Article Title: Liquid biopsy: paving a new avenue for cancer research
    Article Snippet: Counterstain markers targeting non-CTC cells, such as white blood cells and red blood cells, have also been used to enrich CTCs from blood samples, and technologies such as the EasySep depletion kit (StemCell Technologies) have been developed for this purpose. .. Additional approaches, such as the RosetteSep method (StemCell Technologies), incorporate an extra step involving density gradient centrifugation to enhance CTC enrichment. ..

    Selection:

    Article Title: A seven-gene expression panel distinguishing clonal expansions of pre-leukemic and chronic lymphocytic leukemia B cells from normal B lymphocytes.
    Article Snippet: Chronic lymphocytic leukemia (CLL) is a clonal disease of B lymphocytes manifesting as an absolute lymphocytosis in the blood.. However, not all lymphocytoses are leukemic.. In addition, first-degree relatives of CLL patients have an *15 % chance of developing a precursor condition to CLL termed monoclonal B cell lymphocytosis (MBL), and distinguishing CLL and MBL B lymphocytes from normal B cell expansions can be a challenge.

    Isolation:

    Article Title: A seven-gene expression panel distinguishing clonal expansions of pre-leukemic and chronic lymphocytic leukemia B cells from normal B lymphocytes.
    Article Snippet: Chronic lymphocytic leukemia (CLL) is a clonal disease of B lymphocytes manifesting as an absolute lymphocytosis in the blood.. However, not all lymphocytoses are leukemic.. In addition, first-degree relatives of CLL patients have an *15 % chance of developing a precursor condition to CLL termed monoclonal B cell lymphocytosis (MBL), and distinguishing CLL and MBL B lymphocytes from normal B cell expansions can be a challenge.

    Purification:

    Article Title: Characterization of KIR + NK cell subsets with a monoclonal antibody selectively recognizing KIR2DL1 and blocking the specific interaction with HLA‐C
    Article Snippet: .. NK cells, purified using the RosetteSep method (StemCell Technologies, Vancouver, BC), were cultured on irradiated feeder cells in the presence of 2 μg/ml phytohemagglutinin (Sigma‐Aldrich, Irvine, UK) and 600 IU/ml rIL‐2 (Proleukin, Chiron Corp., Emeryville, USA) to obtain proliferation and expansion of activated polyclonal NK cells. ..

    Article Title: Epitope characterization of a monoclonal antibody that selectively recognizes KIR2DL1 allotypes
    Article Snippet: .. NK cells were purified using the RosetteSep method (StemCell Technologies, Vancouver, BC) and cultured on irradiated feeder cells in the presence of 2 μg/ml phytohemagglutinin (Sigma‐Aldrich, Irvine, UK) and 600 IU/ml rIL‐2 (Proleukin, Chiron Corp., Emeryville, CA) to obtain activated polyclonal NK cells. .. NK cells were stained first with HP‐DM1 mAb followed by anti‐IgG1‐PE (Southern Biotechnology, Birmingham, AL), washed twice, and incubated with EB6B‐APC (anti‐KIR2DL1/S1 and anti‐KIR2DL3 allotypes carrying E35 and R50, Beckman Coulter, Brea, CA).

    Cell Culture:

    Article Title: Characterization of KIR + NK cell subsets with a monoclonal antibody selectively recognizing KIR2DL1 and blocking the specific interaction with HLA‐C
    Article Snippet: .. NK cells, purified using the RosetteSep method (StemCell Technologies, Vancouver, BC), were cultured on irradiated feeder cells in the presence of 2 μg/ml phytohemagglutinin (Sigma‐Aldrich, Irvine, UK) and 600 IU/ml rIL‐2 (Proleukin, Chiron Corp., Emeryville, USA) to obtain proliferation and expansion of activated polyclonal NK cells. ..

    Article Title: Epitope characterization of a monoclonal antibody that selectively recognizes KIR2DL1 allotypes
    Article Snippet: .. NK cells were purified using the RosetteSep method (StemCell Technologies, Vancouver, BC) and cultured on irradiated feeder cells in the presence of 2 μg/ml phytohemagglutinin (Sigma‐Aldrich, Irvine, UK) and 600 IU/ml rIL‐2 (Proleukin, Chiron Corp., Emeryville, CA) to obtain activated polyclonal NK cells. .. NK cells were stained first with HP‐DM1 mAb followed by anti‐IgG1‐PE (Southern Biotechnology, Birmingham, AL), washed twice, and incubated with EB6B‐APC (anti‐KIR2DL1/S1 and anti‐KIR2DL3 allotypes carrying E35 and R50, Beckman Coulter, Brea, CA).

    Irradiation:

    Article Title: Characterization of KIR + NK cell subsets with a monoclonal antibody selectively recognizing KIR2DL1 and blocking the specific interaction with HLA‐C
    Article Snippet: .. NK cells, purified using the RosetteSep method (StemCell Technologies, Vancouver, BC), were cultured on irradiated feeder cells in the presence of 2 μg/ml phytohemagglutinin (Sigma‐Aldrich, Irvine, UK) and 600 IU/ml rIL‐2 (Proleukin, Chiron Corp., Emeryville, USA) to obtain proliferation and expansion of activated polyclonal NK cells. ..

    Article Title: Epitope characterization of a monoclonal antibody that selectively recognizes KIR2DL1 allotypes
    Article Snippet: .. NK cells were purified using the RosetteSep method (StemCell Technologies, Vancouver, BC) and cultured on irradiated feeder cells in the presence of 2 μg/ml phytohemagglutinin (Sigma‐Aldrich, Irvine, UK) and 600 IU/ml rIL‐2 (Proleukin, Chiron Corp., Emeryville, CA) to obtain activated polyclonal NK cells. .. NK cells were stained first with HP‐DM1 mAb followed by anti‐IgG1‐PE (Southern Biotechnology, Birmingham, AL), washed twice, and incubated with EB6B‐APC (anti‐KIR2DL1/S1 and anti‐KIR2DL3 allotypes carrying E35 and R50, Beckman Coulter, Brea, CA).



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    Inhibition of ERAP1 enhances <t>NK</t> <t>cell</t> killing of 221-B*51:01 cells. (A) , Example of degranulation by human CD56 + <t>NK</t> <t>cells</t> from one healthy donor expressing KIR2DL1, KIR2DL3, and KIR3DL1 measured as CD107a cell surface expression in response to the indicated cells. The percentage of CD107a + cells within the indicated NK cell subsets is shown. (B) Summary of NK cell degranulation from donors after stimulation with 221 parental and transfectant cells in response to ERAP1 inhibition. P values, calculated by two-tailed paired Student’s t -test. ns, not significant.
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    Inhibition of ERAP1 enhances NK cell killing of 221-B*51:01 cells. (A) , Example of degranulation by human CD56 + NK cells from one healthy donor expressing KIR2DL1, KIR2DL3, and KIR3DL1 measured as CD107a cell surface expression in response to the indicated cells. The percentage of CD107a + cells within the indicated NK cell subsets is shown. (B) Summary of NK cell degranulation from donors after stimulation with 221 parental and transfectant cells in response to ERAP1 inhibition. P values, calculated by two-tailed paired Student’s t -test. ns, not significant.

    Journal: Frontiers in Immunology

    Article Title: ERAP1 Controls the Interaction of the Inhibitory Receptor KIR3DL1 With HLA-B51:01 by Affecting Natural Killer Cell Function

    doi: 10.3389/fimmu.2021.778103

    Figure Lengend Snippet: Inhibition of ERAP1 enhances NK cell killing of 221-B*51:01 cells. (A) , Example of degranulation by human CD56 + NK cells from one healthy donor expressing KIR2DL1, KIR2DL3, and KIR3DL1 measured as CD107a cell surface expression in response to the indicated cells. The percentage of CD107a + cells within the indicated NK cell subsets is shown. (B) Summary of NK cell degranulation from donors after stimulation with 221 parental and transfectant cells in response to ERAP1 inhibition. P values, calculated by two-tailed paired Student’s t -test. ns, not significant.

    Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMC) of healthy donors co-expressing KIR2DL1, KIR2DL2 and KIR3DL1 by using the RosetteSep NK cell enrichment mixture method (Stem-Cell Technologies).

    Techniques: Inhibition, Expressing, Transfection, Two Tailed Test

    Inhibition of ERAP1 enhanced NK cell killing of 221-B*51:01 cells by affecting the engagement of KIR3DL1. (A) , Example of degranulation by human CD56 + NK cells from one healthy donor co-expressing KIR2DL1, KIR2DL3, and KIR3DL1; CD107a cell surface expression was measured in specific KIR single positive (KIRsp) subsets (KIR3DL1sp for 221-B51 cells, KIR2DL1sp for 221-Cw4 cells and KIR2DL3 for 221-Cw3 and -Cw7 cells) in response to ERAP1-inhibited 221 transfectants and control cells. The percentage of CD107a + cells within the indicated NK cell subsets is shown. (B) , Summary of NK cell degranulation in the indicated KIRsp NK subsets from healthy donors after stimulation with 221 parental and transfectant cells in response to ERAP1 inhibition. P values, calculated by two-tailed paired Student’s t -test. ns, not significant.

    Journal: Frontiers in Immunology

    Article Title: ERAP1 Controls the Interaction of the Inhibitory Receptor KIR3DL1 With HLA-B51:01 by Affecting Natural Killer Cell Function

    doi: 10.3389/fimmu.2021.778103

    Figure Lengend Snippet: Inhibition of ERAP1 enhanced NK cell killing of 221-B*51:01 cells by affecting the engagement of KIR3DL1. (A) , Example of degranulation by human CD56 + NK cells from one healthy donor co-expressing KIR2DL1, KIR2DL3, and KIR3DL1; CD107a cell surface expression was measured in specific KIR single positive (KIRsp) subsets (KIR3DL1sp for 221-B51 cells, KIR2DL1sp for 221-Cw4 cells and KIR2DL3 for 221-Cw3 and -Cw7 cells) in response to ERAP1-inhibited 221 transfectants and control cells. The percentage of CD107a + cells within the indicated NK cell subsets is shown. (B) , Summary of NK cell degranulation in the indicated KIRsp NK subsets from healthy donors after stimulation with 221 parental and transfectant cells in response to ERAP1 inhibition. P values, calculated by two-tailed paired Student’s t -test. ns, not significant.

    Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMC) of healthy donors co-expressing KIR2DL1, KIR2DL2 and KIR3DL1 by using the RosetteSep NK cell enrichment mixture method (Stem-Cell Technologies).

    Techniques: Inhibition, Expressing, Control, Transfection, Two Tailed Test

    Inhibition of ERAP1 enhanced NK cell killing of 221-B*51:01 cells by affecting the engagement of NKG2A. (A) , Example of degranulation by human CD56 + NK cells from one healthy donor; CD107a cell surface expression was measured in NKG2A positive subset negative for KIR3DL1, KIR2DL1 and KIR2DL3 in response to ERAP1-inhibited 221 transfectants and control cells. The percentage of CD107a + cells within the indicated NK cell subset is shown. (B) , Summary of NK cell degranulation in the NKG2A positive KIR negative NK subset from healthy donors after stimulation with 221 parental and transfectant cells in response to ERAP1 inhibition. P values, calculated by two-tailed paired Student’s t -test. ns, not significant.

    Journal: Frontiers in Immunology

    Article Title: ERAP1 Controls the Interaction of the Inhibitory Receptor KIR3DL1 With HLA-B51:01 by Affecting Natural Killer Cell Function

    doi: 10.3389/fimmu.2021.778103

    Figure Lengend Snippet: Inhibition of ERAP1 enhanced NK cell killing of 221-B*51:01 cells by affecting the engagement of NKG2A. (A) , Example of degranulation by human CD56 + NK cells from one healthy donor; CD107a cell surface expression was measured in NKG2A positive subset negative for KIR3DL1, KIR2DL1 and KIR2DL3 in response to ERAP1-inhibited 221 transfectants and control cells. The percentage of CD107a + cells within the indicated NK cell subset is shown. (B) , Summary of NK cell degranulation in the NKG2A positive KIR negative NK subset from healthy donors after stimulation with 221 parental and transfectant cells in response to ERAP1 inhibition. P values, calculated by two-tailed paired Student’s t -test. ns, not significant.

    Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMC) of healthy donors co-expressing KIR2DL1, KIR2DL2 and KIR3DL1 by using the RosetteSep NK cell enrichment mixture method (Stem-Cell Technologies).

    Techniques: Inhibition, Expressing, Control, Transfection, Two Tailed Test

    Genetical and pharmacological inhibition of ERAP1 makes 221-B*51:01 cells more susceptible to lysis by NK cells expressing KIR3DL1*001. (A, B) 221 transfectant cells genetically (A) or pharmacologically (B) inhibited for ERAP1 were incubated with YTS or YTS-KIR3DL1*001 NK cells. A representative experiment of three independently performed is shown. Data represented mean ± SD of triplicate.

    Journal: Frontiers in Immunology

    Article Title: ERAP1 Controls the Interaction of the Inhibitory Receptor KIR3DL1 With HLA-B51:01 by Affecting Natural Killer Cell Function

    doi: 10.3389/fimmu.2021.778103

    Figure Lengend Snippet: Genetical and pharmacological inhibition of ERAP1 makes 221-B*51:01 cells more susceptible to lysis by NK cells expressing KIR3DL1*001. (A, B) 221 transfectant cells genetically (A) or pharmacologically (B) inhibited for ERAP1 were incubated with YTS or YTS-KIR3DL1*001 NK cells. A representative experiment of three independently performed is shown. Data represented mean ± SD of triplicate.

    Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMC) of healthy donors co-expressing KIR2DL1, KIR2DL2 and KIR3DL1 by using the RosetteSep NK cell enrichment mixture method (Stem-Cell Technologies).

    Techniques: Inhibition, Lysis, Expressing, Transfection, Incubation